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cd122 (tm-b1) – fitc  (Thermo Fisher)


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    Structured Review

    Thermo Fisher cd122 (tm-b1) – fitc
    Cd122 (Tm B1) – Fitc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd122+(tm-b1)+%E2%80%93+fitc/pmc10794300-66-12-7
    Average 90 stars, based on 1 article reviews
    cd122 (tm-b1) – fitc - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Helios Expression Is Downregulated on CD8 + Treg in Two Mouse Models of Lupus During Disease Progression
    Article Snippet: .. In the case of flow cytometry for DCs, the cells were harvested in PBS Ethylenediaminetetraacetic acid (EDTA) (5 mM) on day 13, and the following conjugated antibodies were used: B220-efluor450 (RA3-6B2), CCR7-biotin (4B12), CD4-APCef780 (RM4.5), CD8 α -APCef780 (53-6.7), CD11b-Alexaf700 (M1/70), CD11c-APC (N418), CD62L-APC (MEL-14), CD69-PeCy7 (H1.2F3), CD73-PeCy7 (eBioTY/11.8), CD80-PeCy5 (16-10A1), CD122-FITC (fluorescein isothiocyanate) (TM-b1), CD127-PeCy7 (A7R34), FoxP3-PE (FJK-16s), Helios-ef450 (22F6), inducible co-stimulator (ICOS)-FITC (7E.17G9), Ly49-PE (14B11), PDCA-1-PeCy7 (eBio927), RORγ(t)-PE (AFKJS-9), and TCRVα2-PE (B20.1, all from eBioscience); B220-PeCy7 (RA3-6B2), CD3-APC (145-2C11), CD3-PercP (145-2C11), CD4-APC (RM4.5), CD8 α -APC (53-6.7), CD8 α -PercP (53-6.7), CD44-PercP (IM7), CD49b-PE (DX5), CD86-PE (GL-1), CD335(NKp46)-APC (29A1.4), Major Histocompatibility Complex Class II (MHCII)-PE (M5/114.15.2), and PDL-1 (10F.9G2, all from BioLegend); CD11c-FITC (HL3), CD25-biotin (7D4), CD62L-PE (MEL-14), CD244.2-biotin (2B4), TCRβ-PE (H57-597), and TCRγδ-biotin (GL3, all from BD Biosciences); CD3-FITC (145-2C11), CD19-SPRD (6D5), and CD44-FITC (KM201, all from SouthernBiotech); CD244.1-biotin (REA524, Miltenyi Biotec). .. Secondary biotin staining was achieved with Streptavidin-APC (Beckman Coulter), Streptavidin-APCCy7 (BioLegend), or streptavidin-PercPef710 (eBioscience).

    Immunopeptidomics:

    Article Title: Helios Expression Is Downregulated on CD8 + Treg in Two Mouse Models of Lupus During Disease Progression
    Article Snippet: .. In the case of flow cytometry for DCs, the cells were harvested in PBS Ethylenediaminetetraacetic acid (EDTA) (5 mM) on day 13, and the following conjugated antibodies were used: B220-efluor450 (RA3-6B2), CCR7-biotin (4B12), CD4-APCef780 (RM4.5), CD8 α -APCef780 (53-6.7), CD11b-Alexaf700 (M1/70), CD11c-APC (N418), CD62L-APC (MEL-14), CD69-PeCy7 (H1.2F3), CD73-PeCy7 (eBioTY/11.8), CD80-PeCy5 (16-10A1), CD122-FITC (fluorescein isothiocyanate) (TM-b1), CD127-PeCy7 (A7R34), FoxP3-PE (FJK-16s), Helios-ef450 (22F6), inducible co-stimulator (ICOS)-FITC (7E.17G9), Ly49-PE (14B11), PDCA-1-PeCy7 (eBio927), RORγ(t)-PE (AFKJS-9), and TCRVα2-PE (B20.1, all from eBioscience); B220-PeCy7 (RA3-6B2), CD3-APC (145-2C11), CD3-PercP (145-2C11), CD4-APC (RM4.5), CD8 α -APC (53-6.7), CD8 α -PercP (53-6.7), CD44-PercP (IM7), CD49b-PE (DX5), CD86-PE (GL-1), CD335(NKp46)-APC (29A1.4), Major Histocompatibility Complex Class II (MHCII)-PE (M5/114.15.2), and PDL-1 (10F.9G2, all from BioLegend); CD11c-FITC (HL3), CD25-biotin (7D4), CD62L-PE (MEL-14), CD244.2-biotin (2B4), TCRβ-PE (H57-597), and TCRγδ-biotin (GL3, all from BD Biosciences); CD3-FITC (145-2C11), CD19-SPRD (6D5), and CD44-FITC (KM201, all from SouthernBiotech); CD244.1-biotin (REA524, Miltenyi Biotec). .. Secondary biotin staining was achieved with Streptavidin-APC (Beckman Coulter), Streptavidin-APCCy7 (BioLegend), or streptavidin-PercPef710 (eBioscience).

    other:

    Article Title: Antigen receptor-mediated depletion of FOXP3 in induced regulatory T-lymphocytes via PTPN2 and FOXO1
    Article Snippet: The three chains of the IL-2R were extracellularly stained using the antibodies Tm-b1-FITC against CD122 (5 μg ml −1 ), TUGm2-Biotin against CD132 (1 μg ml −1 ) and PC61.5-APC against CD25 (50 ng ml −1 ) (all by eBioscience).



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    Image Search Results


    ( a ) Western blot for pSTAT5, STAT5 and β-actin in lysates of iTregs re-cultured for 24 h under the indicated conditions, deprived of IL-2 for 1 h and resupplied with IL-2 (200 U ml −1 ) for 10 min. ( b , c ) Staining of surface CD25, CD122 or CD132 (open areas) vs isotype controls (grey areas) or of FOXP3 on iTreg re-cultured for 48 h in the presence of IL-2 ( b : 20 U ml −1 ; c : 1 U ml −1 ) with or without αCD3. ( a ) Four, ( b , c ) two experiments with similar outcome.

    Journal: Nature Communications

    Article Title: Antigen receptor-mediated depletion of FOXP3 in induced regulatory T-lymphocytes via PTPN2 and FOXO1

    doi: 10.1038/ncomms9576

    Figure Lengend Snippet: ( a ) Western blot for pSTAT5, STAT5 and β-actin in lysates of iTregs re-cultured for 24 h under the indicated conditions, deprived of IL-2 for 1 h and resupplied with IL-2 (200 U ml −1 ) for 10 min. ( b , c ) Staining of surface CD25, CD122 or CD132 (open areas) vs isotype controls (grey areas) or of FOXP3 on iTreg re-cultured for 48 h in the presence of IL-2 ( b : 20 U ml −1 ; c : 1 U ml −1 ) with or without αCD3. ( a ) Four, ( b , c ) two experiments with similar outcome.

    Article Snippet: The three chains of the IL-2R were extracellularly stained using the antibodies Tm-b1-FITC against CD122 (5 μg ml −1 ), TUGm2-Biotin against CD132 (1 μg ml −1 ) and PC61.5-APC against CD25 (50 ng ml −1 ) (all by eBioscience).

    Techniques: Western Blot, Cell Culture, Staining